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Characterization of Glossy1-Homologous Genes of Rice Involved in Leaf Wax Accumulation and Drought Resistance

Asadul Islam  
【摘要】: The vital importance of plant surface wax in protecting tissue from environmental stresses is reflected in the huge commitment of epidermal cells to cuticle formation. Glossy 1 (GL1) is one of the reported genes controlling wax synthesis. This study aimed to identify GL1-homologous genes in Oryza sativa and characterize the key members of this family involved in wax synthesis and stress resistance. Sequence analysis revealed 11 homologous genes of GL1 in rice, designated OsGL1-1-OsGL1-11. OsGL1-1,-2 and-3 are closely related to GL1. OsGL1-4,-5,-6, and -7 are closely related to Arabidopsis CER1 that is involved in cuticular wax biosynthesis. OsGL1-8,-9,-10 and -11 are closely related to SUR2 encoding a putative sterol desaturase also involved in epicuticular wax biosynthesis. These genes showed variable expression levels in different tissues and organs of rice, and most of them were induced by abiotic stresses. Compared to the wild type, the OsGL1-2-over-expression rice exhibited more wax crystallization and a thicker epicuticular layer; while the mutant of this gene showed less wax crystallization and a thinner cuticular layer. Chlorophyll leaching and water loss experiment suggested that the cuticular permeability was decreased and increased in the over-expression lines and the mutant, respectively. To identify additional components involved in cuticular wax production, the role of the OsGL1-2 gene, defective in the osgl1-2 mutant were investigated. Quantification analysis of wax composition by GC-MS revealed a significant reduction of total cuticular wax in the mutant and increase of total cuticular wax in the over-expression plants. Compared to the over-expression and wild type plants, the osgl1-2 mutant was more sensitive to drought stress at reproductive stage, suggesting an important role of this gene in drought resistance.

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1 ;Increased sensitivity to drought stress in atlon4 Arabidopsis mutant[J];Chinese Science Bulletin;2010年32期
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3 ;Characterization of a new mutant allele of the Arabidopsis Flowering Locus D (FLD) gene that controls theflowering time by repressing FLC[J];Chinese Science Bulletin;2005年23期
4 ;Arabidopsis F-box gene FOA1 involved in ABA signaling[J];Science China(Life Sciences);2012年06期
5 ;Insight into herbicide resistance of W574L mutant Arabidopsis thaliana acetohydroxyacid synthase:molecular dynamics simulations and binding free energy calculations[J];Science China(Chemistry);2010年01期
6 Kaiming Cao;;Proteomic identification of potential target proteins regulated by an ASK1-mediated proteolysis pathway[J];Cell Research;2006年05期
7 ;Nitric Oxide Regulates Dark-Induced Leaf Senescence Through EIN2 in Arabidopsis[J];Journal of Integrative Plant Biology;2012年08期
8 ;Characterization of variation induced by low-energy N~+ and cloning of differentially expressed cDNA of a mutant in Arabidopsis thaliana[J];Progress in Natural Science;2003年04期
9 ;Functional Analysis of MYB73 of Arabidopsis thaliana Against Bipolaris oryzae[J];Agricultural Sciences in China;2011年05期
10 ;An Arabidopsis mutant atcsr-2 exhibits high cadmium stress sensitivity involved in the restriction of H_2S emission[J];Journal of Zhejiang University-Science B(Biomedicine & Biotechnology);2012年12期
11 ;Gibberellin and Jasmonate Crosstalk during Stamen Development[J];Journal of Integrative Plant Biology;2009年12期
12 ;Regulation of salt and ABA responses by CIPK14, a calcium sensor interacting protein kinase in Arabidopsis[J];Science in China(Series C:Life Sciences);2008年05期
13 ;An Arabidopsis embryonic lethal mutant with reducedexpression of alanyl-tRNA synthetase gene[J];Cell Research;1998年02期
14 ;The Altered Responses of a New Mutant long life span 1 to Cytokinin in Arabidopsis thaliana[J];遗传学报;2012年03期
15 ;Genetic analysis and fine-mapping of a dwarfing with withered leaf-tip mutant in rice[J];遗传学报;2008年12期
16 Shengchun Zhang;Yanli Qi;Ming Liu;Chengwei Yang;;SUMO E3 Ligase AtMMS21 Regulates Drought Tolerance in Arabidopsis thaliana[J];Journal of Integrative Plant Biology;2013年01期
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