收藏本站
《西北农林科技大学》 2010年
收藏 | 手机打开
二维码
手机客户端打开本文

抗条锈病小麦N95175抗性基因的遗传图谱与克隆

Muharam Ali  
【摘要】:Stripe rust caused by Puccinia striiformis f. sp. tritici is one of the most devastating diseases of wheat in China as well as in Pakistan. In the present studies F2 population was established by crossing N95175 resistant to stripe rust race CYR32 with two susceptible lines Huixianhong and Abbondanza to molecularly tag resistance gene existing in wheat line N95175. The segregation of phenotype was accorded with an expected 3:1 ratio in both combinations studied and fit the model of a single dominant gene controlling stripe rust resistance in N95175. Thirty five SSR primer pairs were screened on the parents and bulks and also on individuals since resistance gene to be located in chromosome 1B. The result indicated that most of resistant plants amplified same band as resistant parent while susceptible plants amplified same as susceptible parents studied and considered that markers co-segregated with resistant loci in N95175. This yellow rust resistance gene was considered to be Yr26 originally thought to be also located in chromosome arm IBS linked to marker loci Xgwm273 and Xgwmll with genetic distances ranging from 1.075cM to 2.74cM in both combinations studied. However, the closest loci were observed 2.67cM for Xgwm273 and 1.075cM for Xgwmll in HuixianhongXN95175 and AbbondanzaXN95175 crosses respectively. Hence, it has been concluded that the PCR-based micro satellite markers Xgwm273 and Xgwmll located in chromosome 1B were shown to be very effective for the detection of Yr26 gene in segregating population and can be applied in future wheat breeding strategies. Wheat TaCOPⅡgene was isolated from EST sequence of wheat leaves infected by Puccinia striiformis f. sp. tritici, initially using RT-PCR a primer pair, because available EST sequence was not enough to design two GSP sequences. Following 3'RACE analyses, cDNA fragments around 1865 bp including poly-A signal region at 3'-UTR, was detected by two round RACE-PCR aplications including nested PCR using two sense GSP primers on 1% agarose gel. CAP3 software was used to combine the two fragments into an 1865 bp consensus sequence including a poly-A signal region at 3'-UTR. The sequencing results showed that the 1865 bp sequence shared high similarity to COPII in Arabidopsis thaliana. The region from 35 to 1450 nucleotides was the open reading frame (ORF) encoding a polypeptide of 486 amino acids. The deduced molecular mass of the peptide was 53.378 KDa with a predicted pI of 8.14. The Real Time PCR observation revealed that maximum induction occurred at 24 hpi, among all time points, followed by a slightly increase at 48hpi, while from 72 to 120hpi, the accumulation of transcripts decreased steadily. However, the magnitude of transcripts at 24hpi time point was much higher than that of control Ohpi. It was also observed that all the candidate genes studied were expressed at basal level before Pst inoculation. These results demonstrated that the transcription of TaCOPII gene was expressed at the early stage of stripe rust inoculation, hence suggesting that this gene may participate in host defense response through different regulatory mechanism specialty at early stage of inoculation. Analysis using nulli-tetrasomic Chinese Spring lines further confirmed that the candidate gene TaCOPII was located on chromosome 3A. On the basis of the above results, it is reasonable to assume that the candidate gene TaCOPII was located on the chromosome 3A in wheat line N95175, and its comparison with the other resistance genes located on 3A most of plants suggested that TaCOPII may be involved indirectly to resistance mechanism against stripe rust PST in wheat line N95175. Exploration and utilization of resources of disease resistance genes in native wheat varieties will be helpful both to diversify the resistance genes and to amend the situation of resistance gene simplification in the commercial wheat cultivars. Wheat TaHSP70 fragment, was detected by two RACE-PCR applications including nested PCR using two gene specific sense primers (GSP). Following plasmid construction, transformation and cDNA clones sequencing,3'RACE fragment was obtained by removing vector and adapter sequences. The rest of about 1203 bp sequence was obtained by RT-PCR using a primer pair. Furthermore, CAP3 software was used to combine the two fragments into an 1810 bp consensus sequence including a poly-A signal region in the 3'-UTR. BLAST analysis showed that the 1810 bp sequence shared high similarity (94%) to Arbidopsis thaliana. The region from 43 to 960 nucleotides was the open reading frame (ORF) encoding a polypeptide of 305 amino acids. The deduced molecular mass of the peptide was 31.31 KDa with a predicted pI of 5.29. Based on the strong identity to A. thaliana, hence putatively designated the wheat 1810bp sequence as TaHSP70. Nulli-tetrasomic Analysis using Chinese Spring lines further confirmed that the candidate gene TaHSP70 was located on chromosome 4A. The Real Time PCR results demonstrated that the transcription of TaHSP70 gene was down regulated at the early stage of stripe rust inoculation hence concluded that this gene may not participate in host defence regulatory mechanism specialty at early stage of inoculation.
【学位授予单位】:西北农林科技大学
【学位级别】:博士
【学位授予年份】:2010
【分类号】:S512.1

手机知网App
【参考文献】
中国期刊全文数据库 前8条
1 王长有;吉万全;张改生;王秋英;蔡东明;薛秀庄;;小麦种质N9134抗白粉病基因的SSR标记和染色体初步定位(英文)[J];作物学报;2007年01期
2 刘天明;胡银岗;张宏;宋国琦;林凡云;吉万全;;条锈菌诱导的抗锈小麦种质的基因表达分析[J];西北植物学报;2006年03期
3 曹张军,王美南,井金学;小麦抗条锈性遗传研究进展[J];麦类作物学报;2001年03期
4 陈佩度,刘大钧,齐莉莉,周波,张守忠,盛宝钦,段霞瑜,王保通,金欣藻,刘正德,黄光明,蒋滨;小麦条锈病新抗源的抗谱鉴定初析[J];植物病理学报;2001年01期
5 吴立人,牛永春;我国小麦条锈病持续控制的策略[J];中国农业科学;2000年05期
6 王凤乐,吴立人,徐世昌,杨家秀,刘述英;绵阳系小麦抗条锈性变异的系统调查[J];植物病理学报;1996年02期
7 汪可宁;洪锡午;司权民;王剑雄;沈谨朴;;我国小麦条锈菌生理专化研究[J];植物保护学报;1963年01期
8 陆师义,范桂芳,謝淑敏,吳維中,孔显良,楊作民,汪可宁,李瑞碧;小麦条绣病研究——Ⅰ.小麦条锈菌的专化性研究[J];植物病理学报;1956年02期
【共引文献】
中国期刊全文数据库 前10条
1 樊玉;薛楠;李高宝;李强;王保通;;2013年鄂西北小麦条锈菌群体毒性结构分析[J];麦类作物学报;2015年12期
2 叶青松;肖能武;张凡;蔡高磊;杨立军;;十堰麦区小麦条锈病精准监测及药剂防治的初步研究[J];湖北农业科学;2015年22期
3 赵宁娟;孟庆立;张宇文;张华锋;屈洋;胡银岗;;利用SSR标记分析44份玉米自交系的遗传多样性[J];种子;2015年11期
4 黄苗苗;李亚凯;黄瑾;贾秋珍;孙振宇;张勃;王晓明;王万军;曹世勤;金社林;;冬小麦品种‘兰天23号’苗期抗条锈性遗传分析[J];植物保护;2015年05期
5 叶青松;肖能武;张凡;蔡高磊;杨立军;向礼波;;湖北十堰麦区小麦条锈菌越夏和越冬研究[J];麦类作物学报;2015年09期
6 李邦发;;西科麦2028抗条锈性的遗传分析[J];植物遗传资源学报;2015年05期
7 韩强;贺雪莲;卓仁英;何正权;;植物耐盐基因筛选方法及其转基因育种研究[J];湖北农业科学;2015年13期
8 杨悦;;广元市小麦条锈病菌源地综合治理技术研究与应用[J];陕西农业科学;2015年04期
9 黄保全;张勇;刘刚;张康;高霞;;汉中市小麦实施“一喷三防”技术思考[J];陕西农业科学;2015年04期
10 周新力;詹刚明;黄丽丽;韩德俊;康振生;;80份国外春小麦种质资源抗条锈性评价[J];中国农业科学;2015年08期
【二级参考文献】
中国期刊全文数据库 前10条
1 刘春燕,王伟权,陈庆山,杨翠平,李文滨,辛大伟,金振国,宋英博;大豆花叶病毒胁迫诱导的消减文库构建及初步分析[J];生物工程学报;2005年02期
2 孔凡娜,曹张军,井金学;DNA分子标记在小麦抗条锈性遗传研究中的应用[J];西北植物学报;2002年05期
3 骆蒙,孔秀英,霍纳新,周荣华,贾继增;小麦抗白粉病侵染初期的表达序列标签分析[J];遗传学报;2002年06期
4 曹张军,王美南,井金学;小麦抗条锈性遗传研究进展[J];麦类作物学报;2001年03期
5 井金学,傅杰,袁红旭,王美南,商鸿生,李振岐;三个小麦野生近缘种抗条锈性传递的初步研究[J];植物病理学报;1999年02期
6 高胜国;南农92R系统白粉病抗源多抗性鉴定及其抗条锈性遗传分析[J];作物学报;1999年03期
7 牛永春,刘红彦,吴立人,徐世昌;小麦品种“Lee”中抗条锈病基因的 PAPD 标记[J];高技术通讯;1998年12期
8 朱桂清,曹远银,姚平,王小奇,刘维志,王铁梁,朱庆顺;小麦抗秆锈病基因推导的计算机程序及应用[J];沈阳农业大学学报;1998年03期
9 商鸿生;小麦对条锈病的高温抗病性研究[J];中国农业科学;1998年04期
10 王石平,刘克德,王江,张启发;用同源序列的染色体定位寻找水稻抗病基因DNA片段[J];Acta Botanica Sinica;1998年01期
【相似文献】
中国期刊全文数据库 前10条
1 Jemima Brinton;Cristobal Uauy;;A reductionist approach to dissecting grain weight and yield in wheat[J];Journal of Integrative Plant Biology;2019年03期
2 ;A Transient Expression System for the Functional Assessment of Early Response Genes on the Powdery Mildew Infected Barley or Wheat Leaves[J];Agricultural Sciences in China;2003年10期
3 ZHANG Pei-pei;Takele Weldu Gebrewahid;ZHOU Yue;LI Qing-luo;LI Zai-feng;LIU Da-qun;;Seedling and adult plant resistance to leaf rust in 46 Chinese bread wheat landraces and 39 wheat lines with known Lr genes[J];Journal of Integrative Agriculture;2019年05期
4 XU Xiao-dan;FENG Jing;FAN Jie-ru;LIU Zhi-yong;LI Qiang;ZHOU Yi-lin;MA Zhan-hong;;Identification of the resistance gene to powdery mildew in Chinese wheat landrace Baiyouyantiao[J];Journal of Integrative Agriculture;2018年01期
5 Zhongfu Ni;Hongjian Li;Yue Zhao;Huiru Peng;Zhaorong Hu;Mingming Xin;Qixin Sun;;Genetic improvement of heat tolerance in wheat:Recent progress in understanding the underlying molecular mechanisms[J];The Crop Journal;2018年01期
6 ZOU Ya-fei;QIAO Hong-bo;CAO Xue-ren;LIU Wei;FAN Jie-ru;SONG Yu-li;WANG Bao-tong;ZHOU Yi-lin;;Regionalization of wheat powdery mildew oversummering in China based on digital elevation[J];Journal of Integrative Agriculture;2018年04期
7 LI Qiang;WANG Zheng-rui;LI Ding;WEI Jian-wei;QIAO Wen-chen;MENG Xiang-hai;SUN Shu-luan;LI Hui-min;ZHAO Ming-hui;CHEN Xiu-min;ZHAO Feng-wu;;Evaluation of a new method for quantification of heat tolerance in different wheat cultivars[J];Journal of Integrative Agriculture;2018年04期
8 Yujie LIU;Qiaomin CHEN;Quansheng GE;Junhu DAI;;Spatiotemporal differentiation of changes in wheat phenology in China under climate change from 1981 to 2010[J];Science China(Earth Sciences);2018年08期
9 YAN Qiu-yan;DONG Fei;LOU Ge;YANG Feng;LU Jin-xiu;LI Feng;ZHANG Jian-cheng;LI Jun-hui;DUAN Zeng-qiang;;Alternate row mulching optimizes soil temperature and water conditions and improves wheat yield in dryland farming[J];Journal of Integrative Agriculture;2018年11期
10 YUE Ai-qin;LI Ang;MAO Xin-guo;CHANG Xiao-ping;LI Run-zhi;JING Rui-lian;;Single-nucleotide polymorphisms,mapping and association analysis of 1-FFT-A1 gene in wheat[J];Journal of Integrative Agriculture;2017年04期
中国重要会议论文全文数据库 前10条
1 Maarten Ryder;;Biological control of soil borne diseases of wheat[A];第二届小麦土传病原菌专家研讨班[C];2005年
2 Yue Zhao;Xuejun Tian;Fei Wang;Liyuan Zhang;Mingming Xin;Zhaorong Hu;Yingyin Yao;Zhongfu Ni;Qixin Sun;Huiru Peng;;Characterization of wheat MYB genesresponsive to high temperatures[A];第八届全国小麦基因组学及分子育种大会摘要集[C];2017年
3 Huifang Wang;Zhaorong Hu;Ke Huang;Yao Han;Aiju Zhao;Haiming Han;Long Song;Chaofeng Fan;Run Li;Mingming Xin;Huiru Peng;Yingyin Yao;Qixin Sun;Zhongfu Ni;;Three genomes differentially contribute to the seedling lateral root number in allohexaploid wheat:Evidence from phenotype evolution and gene expression[A];2018全国植物生物学大会论文集[C];2018年
4 Jun Guo;Peng Liu;Yinghui Duan;Jia Guo;Zhensheng Kang;;Crosstalk between the CBL-CIPK and SA signaling pathways in the wheat-Pucciniastriiformis f.sp.tritici pathosystem[A];第六届全国小麦基因组学及分子育种大会论文集[C];2015年
5 ;Functional characterization of a novel antifreeze protein in wheat(Triticumaestivum L.)[A];中国作物学会50周年庆祝会暨2011年学术年会论文集[C];2011年
6 ;TaMDHAR4,a monodehydroascorbate reductase gene in wheat negatively regulates wheat resistance to stripe rust fungus through reactive oxygen metabolism[A];第十三届全国植物基因组学大会论文集[C];2012年
7 Mengjing Zheng;Chen Jin;Yuhua Shi;Yanxia Li;Yanping Yin;Dongqing Yang;Yongli Luo;Dangwei Pang;Xu Xu;Wenqian Li;Jun Ni;Yuanyuan Wang;Zhenlin Wang;Yong Li;;Manipulation of lignin metabolism by plant densities and its relationship with lodging resistance in wheat[A];2016年全国青年作物栽培与生理学术研讨会论文集[C];2016年
8 Jing Wang;Qiang Gao;Yao Han;Haofeng Chen;;Genome-wide SNP discovery and phylogenetic analysis in wheat using genotyping-by-sequencing[A];2016年全国植物生物学大会摘要集[C];2016年
9 Yiping Tong;Hui Li;Mengyun Hu;Xueqiang Zhao;Xue He;Baoyuan Qu;Wenjing Li;;Engineering nitrogen use efficiency in wheat[A];2016年全国植物生物学大会摘要集[C];2016年
10 Wangcang Su;Xiaoli Wang;Hongle Xu;Renhai Wu;Hongdan Hao;;Investigation of the biological characteristics of weedy wheat and its harmful effects on wheat production in seven provinces in China[A];第十三届全国杂草科学大会论文摘要集[C];2017年
中国博士学位论文全文数据库 前10条
1 Muharam Ali;抗条锈病小麦N95175抗性基因的遗传图谱与克隆[D];西北农林科技大学;2010年
2 Shoaib Ur Rehman;小麦蔗糖非发酵相关蛋白激酶2基因TaSnRK2.9的功能分析[D];中国农业科学院;2018年
3 罗培高;小麦对条锈病、白粉病和衰老抗性的分子细胞生物学研究[D];四川农业大学;2006年
4 李玲玲;[D];甘肃农业大学;2006年
5 Muhammad Ejaz Khan;[D];西北农林科技大学;2013年
6 Mohamad Hesam Shahrajabian;华北平原冬小麦灌溉效应及蒸发、蒸腾的田间蒸渗仪评价[D];中国农业大学;2015年
7 Mamoona Hanif;小麦TaTGW6基因的克隆鉴定和功能标记开发[D];中国农业科学院;2016年
8 王丽芳;旱作小麦产量形成及其对不同覆盖与耕作措施的响应[D];西北农林科技大学;2016年
9 Nadia Khan;不同水分条件下小麦茎秆可溶性糖和农艺性状的遗传解析[D];中国农业科学院;2015年
10 Bakht un Nisa Mangan;[D];西北农林科技大学;2014年
中国硕士学位论文全文数据库 前10条
1 Mamoudou Saidou;小麦新种质N0324抗白粉病基因的染色体定位与分子标记[D];西北农林科技大学;2013年
2 Lise Josephine Gerard;对中国小麦市场未来的研究[D];华东理工大学;2015年
3 韩国强;不同施氮条件下小麦品种的碳氮转移效率研究[D];福建农林大学;2010年
4 郑青焕;21份印度小麦种质品质性状的评价及主要农艺性状分析[D];西北农林科技大学;2016年
5 Shahat Sabet MOHAMED;中埃农业比较研究[D];中国农业科学院;2013年
6 刘占锋;利用作物模拟模型辅助冬小麦限水灌溉决策的研究[D];河北农业大学;2004年
7 杨宁;豆科绿肥—冬小麦轮作提高小麦产量和营养元素含量的效应与土壤机制[D];西北农林科技大学;2012年
8 赵富强;鹅观草不同居群条锈病和白粉病抗性评价及抗性遗传分析[D];四川农业大学;2016年
9 罗怀勇;小麦条锈病、白粉病和叶锈病抗病基因的分子标记[D];四川农业大学;2010年
10 张志良;小麦地方品种红蚰麦抗白粉病基因的发掘和定位[D];南京农业大学;2015年
中国知网广告投放
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62791813
  • 010-62985026