收藏本站
收藏 | 手机打开
二维码
手机客户端打开本文

Characterization of a Novel Salt Tolerant Bacterium Gracilibacillus sp. GTY and Its Performance of Dye Decolorization

Salah Uddin  
【摘要】: The main aim of this research work was to decolorize various azo dyes and reactive dyes from lab-scale dye solutions and real textile wastewater samples under high salt concentration conditions. Decolorization experiments were run using a recently isolated halophilic bacterium Gracilibacillus sp. GTY. As a new bacterial strain, some other investigations, such as isolation, characterization and study of a large extrachromosomal DNA were also taken place. So the dissertation is presented in different steps bellow. Firstly, a highly salt tolerant strain obtained from Dalian coastline, was identified as Gracilibacillus sp. GTY on the basis of morphological and physio-biochemical characteristics and 16S rDNA sequence analysis. According to DNA sequence, the maximum 93%-95% homology was found for the strain GTY. The strain was deposited as a patent strain in China General Microorganism Culture Center with the accession number CGMCC 1527. The 16S rDNA sequence of GTY was also submitted to GenBank with the number DQ 286727. The strain was gram-negative, rod-shaped and strictly aerobic. Optimum growth conditions were observed: temperature±30℃, pH 7.2, 10%-15% (w/v) of NaCl, and proline as the best compatible solute as well as a good carbon source. The rapid and smooth growth rates were observed in 10%-15% (w/v) of NaCl supplemented Luria-Bertani (LB) and Mineral-Salt (MS) media, but the strain could survive narrowly in the media containing up to 25% (w/v) of NaCl. So it was considered as a highly salt tolerant strain. Secondly, a large molecule of extrachromosomal DNA was isolated from the strain by a modified method. Then this plasmid DNA was eliminated by acridine orange to know its role in host genome. After successful elimination, it was assumed that the plasmid contains some key genes, which may be responsible for the bacterial salt tolerance. Thirdly, an attempt was made to optimize conditions for the bacterial decolorization of various dyes in lab-scale dye solutions under high salinity conditions. Decolorization experiments were conducted by growing and resting cells as well as azo reductase. Experiments were performed under different concentrations of NaCl. Detailed analytical investigations were performed using only one of the azo dyes, Acid Red B. For growing cells, strain grown in media containing10%-15% (w/v) of NaCl showed the best performance of azo dye Acid Red B decolorization. Dye was almost completely decolorized (nearly 100%) after 96 h of treatment. Strain grown in the media containing 2%-5% (w/v) of NaCl showed very poor decolorization ability where bacterial growth rates were very poor also. On the other hand, strain grown in the media with the highest percentage (25%, w/v) of NaCl showed moderate decolorization ability. Strain grown in lower percentages of NaCl could not show good performance of decolorization because of low growth rates. Decolorization was not satisfactory also in the media containing 25% (w/v) of NaCl, because the inhibition to microorganisms by high salt concentration, which may cause plasmolysis and /or loss of activity of cells. Resting cells also showed the best performance of decolorization in 10%-15% (w/v) of NaCl. Extracted azo reductase was non-purified and non-characterized. The decolorization of dye was conducted in 3 ml of reaction mixture at 30°C in 700 sec. Reaction rate obtained from time course spectra measurement analysis was comparatively slow. The percentage of dye removal was about 10% in the reaction mixture. Finally, several experiments were set up to degrade a textile azo dye Acid Red B and remove COD from a real textile wastewater body. The textile wastewater sample was naturally salty (10% of Na_2SO_4), and COD value was relatively high. The textile wastewater sample was used by adding certain nutrients and NaCl for the proper growth of this salt tolerant strain. The study was conducted on the basis of various environmental factors and substrates. Dye degradation and COD removal rates were comparatively slow in first two days. Decolorization efficiency might be attributed to the microbial shock due to the change of environment and the possible toxic or inhibitory effects of certain compounds of the textile wastewater on the microbe. Nevertheless, after an acclimatization period of 6-10 days, the anaerobic microbes retained their activity leading to wastewater's decolorization efficiency of nearly 96% in optimum growth condition. Then COD removal rate was also satisfied. After decolorization, COD removal was observed 4436.87 (mg/l) where the initial COD value was more than 7000 (mg/l).


知网文化
【相似文献】
中国期刊全文数据库 前20条
1 Qun-chao Wang;Shu-gen Liu;Hua-ping Gao;;Treatment of hydroxyquinone-containing wastewater using precipitation method with barium salt[J];Water Science and Engineering;2019年01期
2 Yang Yu;Zhihong Zou;Shanshan Wang;;Statistical regression modeling for energy consumption in wastewater treatment[J];Journal of Environmental Sciences;2019年01期
3 Ming Liu;Sergei Preis;Iakov Kornev;Yun Hu;Chao-Hai Wei;;Pulsed corona discharge for improving treatability of coking wastewater[J];Journal of Environmental Sciences;2018年02期
4 Ying Wang;Ming Yong;Song Wei;Yuqing Zhang;Wei Liu;Zhiping Xu;;Performance improvement of hybrid polymer membranes for wastewater treatment by introduction of micro reaction locations[J];Progress in Natural Science:Materials International;2018年02期
5 Pengyu Zhu;Kaijin Zhu;Rob Puzey;Xiaoli Ren;;Degradation analysis of A~2/O combined with AgNO_3+K_2FeO_4 on coking wastewater[J];Chinese Journal of Chemical Engineering;2018年07期
6 Tianlong Zheng;Qunhui Wang;Zhining Shi;Yue Fang;Shanshan Shi;Juan Wang;Chuanfu Wu;;Advanced treatment of wet-spun acrylic fiber manufacturing wastewater using three-dimensional electrochemical oxidation[J];Journal of Environmental Sciences;2016年12期
7 Audrey Murray;Banu rmeci;Edward P.C.Lai;;Use of sub-micron sized resin particles for removal of endocrine disrupting compounds and pharmaceuticals from water and wastewater[J];Journal of Environmental Sciences;2017年01期
8 Yawei Xie;Lujun Chen;Rui Liu;;AOX contamination status and genotoxicity of AOX-bearing pharmaceutical wastewater[J];Journal of Environmental Sciences;2017年02期
9 Wang Yan;Zhu Sixi;;Physiological and Biochemical Responses of Zizania latifolia to Traditional Chinese Medicine Wastewater[J];Meteorological and Environmental Research;2017年02期
10 Pradip Gyawali;Warish Ahmed;Jatinder P.Sidhu;Paul Jagals;Simon Toze;;Quantification of hookworm ova from wastewater matrices using quantitative PCR[J];Journal of Environmental Sciences;2017年07期
11 Yiyong Wen;Jianping Yi;Shen Zhao;Song Jiang;Yuming Chi;Kefu Liu;;Non-thermal plasma treatment of Radix aconiti wastewater generated by traditional Chinese medicine processing[J];Journal of Environmental Sciences;2016年06期
12 Xiang Liu;Qiuwen Chen;Liang Zhu;;Improving biodegradation potential of domestic wastewater by manipulating the size distribution of organic matter[J];Journal of Environmental Sciences;2016年09期
13 Dehua Ma;Lujun Chen;Cong Liu;Chenjun Bao;Rui Liu;;Biological removal of antiandrogenic activity in gray wastewater and coking wastewater by membrane reactor process[J];Journal of Environmental Sciences;2015年07期
14 PENG Lin;FENG Chun-xiao;LI Wei;LI De-you;WANG Jian-xin;;Annual Report of Airborne Discharge Station for Purified Tritiated Wastewater in 2016[J];Annual Report of China Institute of Atomic Energy;2016年00期
15 Na Zhu;Hongyan Li;Guangke Li;Nan Sang;;Coking wastewater increases micronucleus frequency in mouse in vivo via oxidative stress[J];Journal of Environmental Sciences;2013年10期
16 王震;叶宽伟;杨哲;夏哲韬;骆沁沁;万先凯;史惠祥;;Magnetic field assisted electrocatalytic oxidation of organic pollutants in electroplating wastewater[J];Journal of Central South University;2012年06期
17 ;Activated carbon supported TiO_2 -photocatalysis doped with Fe ions for continuous treatment of dye wastewater in a dynamic reactor[J];Journal of Environmental Sciences;2010年08期
18 李雨霏;韩洪军;王英伟;方芳;王伟;;Effects of different acclimations on high solution bacteria in coal-gas wastewater degradation[J];Journal of Harbin Institute of Technology;2010年03期
19 ;Advances in chemical technologies for water and wastewater treatment:preface[J];Journal of Environmental Sciences;2009年01期
20 郑雅杰;王勇;肖发新;罗圆;;Recovery of copper sulfate after treating As-containing wastewater by precipitation method[J];Journal of Central South University of Technology;2009年02期
中国重要会议论文全文数据库 前10条
1 Andrzej Kraslawski;;Decision Making for Wastewater Treatment Projects[A];2014年第12届全国水处理化学大会暨学术研讨会论文摘要集[C];2014年
2 ;Occurrence of pharmaceuticals in wastewater treatment plants of Beijing,China[A];持久性有机污染物论坛2009暨第四届持久性有机污染物全国学术研讨会论文集[C];2009年
3 胡张军;肖彦;郜洪文;;Recyclable Sorption Material Obtained by Immobilizing Waste Dye in Barite[A];第五届全国环境化学大会摘要集[C];2009年
4 ;Degradation of Reactive Blue 13 by Wet Air Oxidation[A];持久性有机污染物论坛2010暨第五届持久性有机污染物全国学术研讨会论文集[C];2010年
5 ;The Prediction of Indices at Infall of Confluent Flow Network of Wastewater with Multivariate Linear Regression[A];第二十九届中国控制会议论文集[C];2010年
6 廖文;常园园;吴祖成;;Retrieval and recovery of metal ions from wastewater by use of eletrodeionization process[A];第六届全国环境化学大会暨环境科学仪器与分析仪器展览会摘要集[C];2011年
7 Robert Liu;Ruth Yu-Li Yeh;S.Y.Chung;Y.C.Liu;Yung-tse Hung;;Degradation of FBL Dye by Rare-earth doped Titanium Dioxide[A];中国颗粒学会超微颗粒专委会2013年年会暨第八届海峡两岸超微颗粒学术研讨会论文集[C];2013年
8 Haifeng Chen;Yan Zhou;Liangchao Li;Bei Fan;Xue Wu;Yan Ding;Juanbi Li;;Preparation of magnetic carbon materials and its adsorption for dye wastewater[A];中国化学会2013年中西部地区无机化学化工学术研讨会论文集[C];2013年
9 ;Study and development on innovate integrated technology for wastewater reuse in oilfield[A];第六届化学工程与生物技术展览暨会议论文集[C];2004年
10 ;GA Based LS-SVM Classifier for Waste Water Treatment Process[A];第二十七届中国控制会议论文集[C];2008年
中国博士学位论文全文数据库 前10条
1 贝斯(Haq Nawaz Abbasi);生物生态技术在治理农村水体污染中的应用研究[D];东南大学;2017年
2 Salah Uddin;[D];大连理工大学;2008年
3 童蕾;养猪废水及其处理系统中有机污染物的分布与去除[D];中国地质大学;2010年
4 Ahmed Mohamed Ismaeil Abou-Shady;[D];中国海洋大学;2012年
5 Kouakou Yao Salomon;[D];中国地质大学;2014年
6 刘娜;水解酸化处理印染废水过程中微生物群落结构变化及其优化调控研究[D];东华大学;2016年
7 TiroyaoneTshukudu;聚二烯丙基二甲基氯化铵的合成及其在水处理中的应用[D];重庆大学;2012年
8 Husham Taha Yaseen Ibrahim;缺氧/好氧移动床生物膜反应器处理城市污水的效能研究[D];重庆大学;2014年
9 马利云;非甾体抗炎药的转化及残留检测研究[D];华中科技大学;2017年
10 NGOUYAP MOUAMFON M.Valery;高级氧化工艺降解水溶液中磺胺甲恶唑的研究[D];华东理工大学;2011年
中国硕士学位论文全文数据库 前10条
1 QASIM MUHAMMAD;[D];西南科技大学;2018年
2 SHAIKH ABDULLAH;[D];西南科技大学;2018年
3 韩瑱清;《水生病了吗?废水管理在可持续发展中所起的中心作用》翻译报告[D];四川外语学院;2012年
4 哈桑 穆罕默德;电化学技术处理工业废水的可行性研究[D];华东理工大学;2011年
5 Moutari Abdou Mahamadou Nassirou;[D];河海大学;2006年
6 Umme Marium Ahmad;[D];北京化工大学;2017年
7 陈海星;生态村里供给水—极端条件下水循环和给水研究[D];天津大学;2012年
8 叶徐;生态翻译学视角下《城市环境中水资源的可持续管理》(节选)翻译实践报告[D];南昌大学;2017年
9 路易斯(Luis Vera);基于生命周期评价的玉米淀粉废水处理工艺的经济和环境评价研究[D];北京化工大学;2014年
10 Agus Prihatin;从柑橘罐头废水获得的柑橘果胶作为饼干和冰淇淋中的潜在脂肪代用品[D];浙江大学;2017年
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62982499
  • 010-62783978