1 Objcctivc Tllere is apparent relatio11ship between vascular endothelial
cells aPoptosis and llypertension. The pulPose of this paper was to clarity
whether different pressure conditions affect the proliferation and
apoptosis of cultured human umbi1ical vein endothelial cells, to observe
the protect effects of captopl.i1 on HPC-induced HUVECs apoptosis.
2 Mcthods Cells were identified as HUVECs by their growtll pattern and
morphologic features. Experiments were carried out using cel1s from the
' 4 th through 6th passages. HUVECs were exposed to either atmosphere,
l20, or l80n1mHg pressure by p1acil1g them in a high-tension voltnleter
load with 5%co,/air and maintained in RPMI~ l640 supplemented with
2.5%ca1f seruln and substrates lbr up to 1 ~ 2 days. \Ve invested the
cha1lges of cel1 viability, lnorpho1ogy, agarose gel electrophoresis of
DNA, and flow cytometric a11a1ysis.
3 Results The results indicate that HUVECs pro1iferation is influenced by
middle pressure condition. high pressure condition stimulate proliferation
of HUVECs at 16h, however, at 24h and 48h, cel1 11UInber was
signiticant1y lower. Apoptosis cell was markedly increased under high
pressul'e conditiOn; "DNA Ladder" was obviously seen at 48h. HoweveT,
middle pressure conditiol1 didn't induce apoptosis. An increase in ce1l
viabi1ity and a decrease in apoptosis cel1s were observed in captopril (10-'
' 10-'mol/L) treated HUVECs by a dose-dependence. "DNA Ladder"
disappeared after captopril treatment.
4 Conclusions (l ) Harvest and hiaintenance of HUVECs was perfOrmed-
by modifled Jaffe's culture method.
(2) Ambient pressure in vitro can influence HUVECs
(3 ) High pressure condition can induce cultured HUVECs
apoptosis by a time-dependence.
(4 ) Captopril can decrease or inhibit HUVECs apoptosis
and reverse groWth inhibition of cultured HUVECs
under high pressure condition.